[Na]i and [Ca]i load after ischemia and improves function in intact hearts
نویسندگان
چکیده
JIANZHONG AN, SRINIVASAN G. VARADARAJAN, AMADOU CAMARA, QUN CHEN, ENIS NOVALIJA, GARRETT J. GROSS, AND DAVID F. STOWE Anesthesiology Research Laboratories, Departments of Anesthesiology, Physiology, and Pharmacology and Toxicology, and Cardiovascular Research Center, The Medical College of Wisconsin, Milwaukee 53226; and Research Service, Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295
منابع مشابه
Na(+)/H(+) exchange inhibition with HOE642 improves postischemic recovery due to attenuation of Ca(2+) overload and prolonged acidosis on reperfusion.
BACKGROUND Na(+)/H(+) exchange inhibition with HOE642 (cariporide) improves postischemic recovery of cardiac function, but the mechanisms of action remain speculative. Because Na(+)/H(+) exchange is activated on reperfusion, it was hypothesized that its inhibition delays realkalinization and decreases intracellular Na(+) and, via Na(+)/Ca(2+) exchange, Ca(2+) overload. Attenuated Ca(2+) overloa...
متن کاملNa/H Exchange Inhibition With HOE642 Improves Postischemic Recovery due to Attenuation of Ca Overload and Prolonged Acidosis on Reperfusion
Background—Na/H exchange inhibition with HOE642 (cariporide) improves postischemic recovery of cardiac function, but the mechanisms of action remain speculative. Because Na/H exchange is activated on reperfusion, it was hypothesized that its inhibition delays realkalinization and decreases intracellular Na and, via Na/Ca exchange, Ca overload. Attenuated Ca overload and prolonged acidosis are k...
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Enhanced gene expression of the Na(+)/Ca(2+) exchanger in failing hearts may be a compensatory mechanism to promote influx and efflux of Ca(2+), despite impairment of the sarcoplasmic reticulum (SR). To explore this, we monitored intracellular calcium (Ca(i)(2+)) and cardiac function in mouse hearts engineered to overexpress the Na(+)/Ca(2+) exchanger and subjected to ischemia and hypoxia, cond...
متن کاملChanges in [Na(+)](i), compartmental [Ca(2+)], and NADH with dysfunction after global ischemia in intact hearts.
We measured the effects of global ischemia and reperfusion on intracellular Na(+), NADH, cytosolic and mitochondrial (subscript mito) Ca(2+), relaxation, metabolism, contractility, and Ca(2+) sensitivity in the intact heart. Langendorff-prepared guinea pig hearts were crystalloid perfused, and the left ventricular (LV) pressure (LVP), first derivative of LVP (LV dP/dt), coronary flow, and O(2) ...
متن کاملOverexpression of the Na+/H+ exchanger and ischemia-reperfusion injury in the myocardium.
In the myocardium, the Na(+)/H(+) exchanger isoform-1 (NHE1) activity is detrimental during ischemia-reperfusion (I/R) injury, causing increased intracellular Na(+) (Na(i)(+)) accumulation that results in subsequent Ca(2+) overload. We tested the hypothesis that increased expression of NHE1 would accentuate myocardial I/R injury. Transgenic mice were created that increased the Na(+)/H(+) exchan...
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